生物通报道,清华大学兼职教授颜宁博士和王佳伟副研究员领导的研究小组在ABA(脱落酸受体)结构研究方面取得新进展,相关成果文章Structural Insights into the mechanism of Abscisic acid signaling by PYL proteins发表在Nature系列子刊《Nature Structural & Molecular Biology》在线版上。
生物通报道,清华大学兼职教授颜宁博士和王佳伟副研究员领导的研究小组在ABA(脱落酸受体)结构研究方面取得新进展,相关成果文章Structural Insights into the mechanism of Abscisic acid signaling by PYL proteins发表在Nature系列子刊《Nature Structural & Molecular Biology》在线版上。
Yan N* and Shi Y*. Allosteric activation of a bacterial stress sensor. (Preview) Cell, 2007: 131,441-443.(The single asterisk indicates corresponding authors.)
Feng L, Yan H, Wu Z, Yan N, Wang Z, Jeffrey PD, and ShiY. Structure of a Site-2 Protease Family Intramembrane Metalloprotease. Science, 2007: 318, 1608-1612
Wu Z*, Yan N*, Feng L*, Oberstein A, Yan H, Baker PR, Gu L, Jeffrey PD, Urban S, andShi Y. Structural analysis of a rhomboid family intramembrane protease reveals a gating mechanism for substrate entry. Nat. Struct. & Mol. Biol. 2006: 13, 1084-1091 (The single asterisk indicates equal contributions.)
Yang C, Yan N, Parish J, Wang X, Shi Y, and Xue D. RNA aptamers targeting the cell death inhibitor CED-9 induce cell killing in C. elegans. J. Biol. Chem. 2006: 281(14):9137-44.
Yan N, Huh J-R, Schirf V, Demeler B, Hay B-A, and Shi Y. Structure and activation mechanism of the Drosophila initiator caspase Dronc. J. Biol. Chem. 2006: [Epub ahead of print]
Yan N, Xu Y, and Shi Y. (Perspectives) 2:1 Stoichiometry of the CED-4-CED-9 Complex and the Tetrameric CED-4: Insights into the Regulation of CED-3 Activation. Cell Cycle 2006:5, 31-34
Yan N, Chai J, Lee E-S, Gu L, Liu Q, He J, Wu J, Kokel D, Li H, Hao Q, Xue D, and Shi Y. Structure of the CED-4/CED-9 complex reveals insights into programmed cell death in Caenorhabditis elegans. Nature 2005: 437, 831-837.
Yan N and Shi Y. Mechanisms of Apoptosis through Structural Biology. Annual Review Cell Dev. Biol. 2005: 21, 35-56
Yan N*, Gu L*, Kokel D, Chai J, Li W, Han A, Chen L, Xue D, and Shi Y (2004). Structural, Biochemical and Functional Analyses of CED-9 Recognition by the Pro-apoptotic Proteins EGL-1 and CED-4.Mol. Cell 15, 999–1006. (The single asterisk indicates equal contributions.)
Yan N, Wu J-W, Huh J-R, Chai J, Li W, Hay B-A, and Shi Y.Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid, and Grim.Nature-Structural & Molecular Biology 2004; 11 (5): 420–428.
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Structural insights into the mechanism of abscisic acid signaling by PYL proteins
Abscisic acid (ABA) is an important phytohormone that regulates plant stress responses. Proteins from the PYR-PYL-RCAR family were recently identified as ABA receptors. Upon binding to ABA, a PYL protein associates with type 2C protein phosphatases (PP2Cs) such as ABI1 and ABI2, inhibiting their activity; the molecular mechanisms by which PYLs mediate ABA signaling remain unknown, however. Here we report three crystal structures: apo-PYL2, (+)-ABA-bound PYL2 and (+)-ABA-bound PYL1 in complex with phosphatase ABI1. Apo-PYL2 contains a pocket surrounded by four highly conserved surface loops. In response to ABA binding, loop CL2 closes onto the pocket, creating a surface that recognizes ABI1. In the ternary complex, the CL2 loop is located near the active site of ABI1, blocking the entry of substrate proteins. Together, our data reveal the mechanisms by which ABA regulates PYL-mediated inhibition of PP2Cs.